BIP! Finder - MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation

2021 • MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation

Authors: Vladimir Milovanovic, Rajna Minić, Jelena Vakic, Saša Ivanović, Vitomir Ćupić, Sunčica Borozan, Andrijana Nešić, Irena Zivkovic

Venue: Toxicon

Type: Publication

Abstract: The MTT assay is routinely used to detect the activity of living cells. While working with Vipera ammodytes venom we detected the reduction of MTT without the presence of cells, in a concentration-dependent manner. By combining non-reducing PAGE, L-amino acid oxidase (LAAO) assays, and standard MTT assays, we established and confirmed that venom MTT reduction is catalyzed by only one enzyme, the LAAO. Even though it was previously known that the dimeric and tetrameric forms of LAAO are active, we conclude that the enzyme is also active in the m... (read more)

Topics: Molecular biology Biochemistry

DOI: 10.1016/j.toxicon.2021.01.012

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